They conducted a preclinical study that demonstrated the survival of human renal epithelial cells (HRECs) within an implantable bioreactor in pigs. These cells were able to replicate important kidney functions without requiring systemic immune suppression (
The study is a significant advancement for The Kidney Project, led by Shuvo Roy, PhD, from UCSF, and William H. Fissell, MD, from Vanderbilt University Medical Center. The research focused on establishing the feasibility of a bioreactor component for an implantable bioartificial kidney (iBAK), which employs silicon nanopore membranes (SNPs) to protect the cells from immune rejection.
Bioreactor with Kidney Cells
The ultimate goal is to populate the bioreactor with various kidney cells responsible for essential functions like fluid balance and hormone regulation and combine it with a hemofilter device for blood waste filtration.
The researchers emphasized their commitment to replicating key kidney functions safely. The bioartificial kidney they’re developing is anticipated to improve kidney disease treatment, making it more effective, comfortable, and tolerable.
The researchers detailed their progress in a paper titled “Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes,” published in Nature Communications. The study marks a step toward developing an iBAK with a bioreactor component that mimics native renal tubule function. They demonstrated the feasibility of a bioreactor using SNPs to potentially protect against rejection and sustain HRECs both in vitro and in vivo.
Over two million individuals globally receive treatment for end-stage renal disease (ESRD), a number rising due to diabetes and hypertension rates. While kidney transplantation offers positive outcomes, the shortage of donors and the need for lifelong immunosuppression pose challenges. The Kidney Project aims to address these issues by creating a permanent bioartificial kidney solution.
The team designed a bioreactor that interfaces directly with blood vessels, allowing nutrient and oxygen exchange, similar to a transplanted kidney. The silicon nanopore membranes prevent immune cell attacks on the kidney cells. The researchers utilized proximal tubule cells, which regulate water, for testing. These cells have been successfully used by co-author H. David Humes, MD, to aid dialysis patients.
Hawk-Eyeing the Transplanted Kidney
The team monitored the transplanted kidney cells and recipient animals for a week, observing positive outcomes. The bioreactor prototypes containing SNMs remained functional in pigs without systemic immune suppression. The researchers acknowledged that the design prioritized cell viability over transport or metabolic function. Nonetheless, their findings demonstrated the feasibility of an implantable bioreactor with silicon nanopore membranes for protecting human renal epithelial cells.
The researchers plan to conduct month-long animal trials, eventually progressing to human trials. They are confident in their approach, having demonstrated the functionality of the bioreactor without the need for immune-suppressing drugs. The next steps involve expanding cell numbers and implantation durations to establish definitive proof and statistical significance.
In conclusion, the study represents a promising advancement in kidney disease treatment, potentially paving the way for a bioartificial kidney that can replicate essential functions while minimizing the need for immune suppression drugs. In a recent study, a pig kidney made a 32-day mark in a human recipient. All these studies are definitely a stepping stone to finding a permanent solution for patients suffering from kidney diseases.
- Can an Artificial Kidney Finally Free Patients from Dialysis? – (https:www.ucsf.edu/news/2023/08/426056/can-artificial-kidney-finally-free-patients-dialysis)